Recombinant Human IFNA5 protein(Leu22-Glu189), hFc-tagged

• Cat.No.: IFNA5-30H
• Product Overview: Recombinant Human IFNα G (NP_002160.1) (Leu 22-Glu 189) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Leu22-Glu189
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is 0.16-0.8 ng/mL.
• Molecular Mass: The recombinant human IFNα G/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 405 amino acids with a predicted molecular mass of 46.3 kDa. As a result of glycosylation, rh IFNα G/Fc monomer migrates as an approximately 48-50 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IFNA5 interferon, alpha 5 [ Homo sapiens ]
• Official Symbol: IFNA5
• Synonyms: IFNA5; interferon, alpha 5; interferon alpha-5; IFN alphaG; leIF G; IFN-alpha-5; interferon alpha-G; interferon alpha-61; INFA5; IFN-alphaG
• Gene ID: 3442
• mRNA Refseq: NM_002169
• Protein Refseq: NP_002160
• MIM: 147565
• UniProt ID: P01569