Recombinant Human IGFBP3 protein(Gly28-Lys291), His-tagged
Cat.No. : | IGFBP3-758H |
Product Overview : | Recombinant Human IGFBP3 (NP_000589.2) (Gly28-Lys291) was expressed in HEK293, fused with a polyhistidine tag at the N-terminus. |
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Source : | HEK293 |
Species : | Human |
Tag : | N-His |
Protein length : | Gly28-Lys291 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized human IGF2 at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 18 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized human IGF1 at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC50 of biotinylated Human His-IGFBP3 is 24 ng/mL. 3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.05-0.2 μg/mL in the presence of 14 ng/mL human IGFII. |
Molecular Mass : | The secreted recombinant human IGFBP3 comprises 280 amino acids with a predicted molecular mass of 31 kDa. As a result of glycosylation, the apparent molecular mass of rhIGFBP3 is approximately 40-45 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 85 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | IGFBP3 insulin-like growth factor binding protein 3 [ Homo sapiens ] |
Official Symbol : | IGFBP3 |
Synonyms : | IGFBP3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; BP 53; growth hormone dependent binding protein; IBP3; IGF binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; growth hormone-dependent binding protein; BP-53; |
Gene ID : | 3486 |
mRNA Refseq : | NM_000598 |
Protein Refseq : | NP_000589 |
MIM : | 146732 |
UniProt ID : | P17936 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, genetic variations can affect IGFBP3 expression, potentially leading to differences in protein levels among individuals.
IGFBP3 is involved in bone metabolism and is associated with bone mineral density. Low levels of IGFBP3 have been linked to decreased bone density and increased risk of fractures.
The potential side effects of therapies targeting IGFBP3 are still being studied, and it varies depending on the specific treatment approach being used.
Healthy lifestyle choices, including a balanced diet and regular exercise, can positively influence IGFBP3 levels, promoting overall health and reducing the risk of related diseases.
IGFBP3 deficiency can impact children's growth and development, potentially leading to short stature and delayed puberty, as IGFBP3 is a key regulator of growth hormone actions.
Customer Reviews (3)
Write a reviewA manufacturer that offers customization options can enable the researcher to adhere to their experimental design requirements more effectively.
A reliable manufacturer should be able to provide a consistently pure and high-quality IGFBP3 protein, ensuring that the samples are suitable for research purposes.
This can help ensure compatibility between different components used in the trials and ease the overall experimental workflow.
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