Recombinant Human IGFBP3 protein, His-tagged

• Cat.No.: IGFBP3-828H
• Product Overview: Recombinant Human IGFBP3 aa. (Gly135~Thr277 (Accession # P17936)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 17.5kDa
• Protein length: Gly135~Thr277 (Accession # P17936)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 9.3.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: IGFBP3 insulin like growth factor binding protein 3 [ Homo sapiens (human) ]
• Official Symbol: IGFBP3
• Synonyms: IGFBP3; insulin like growth factor binding protein 3; IBP3; BP-53; insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein
• Gene ID: 3486
• mRNA Refseq: NM_000598.4
• Protein Refseq: NP_000589.2
• UniProt ID: P17936

Reviews

05/04/2020

A manufacturer that offers customization options can enable the researcher to adhere to their experimental design requirements more effectively.

01/20/2019

A reliable manufacturer should be able to provide a consistently pure and high-quality IGFBP3 protein, ensuring that the samples are suitable for research purposes.

01/02/2019

This can help ensure compatibility between different components used in the trials and ease the overall experimental workflow.

Q&As

Are there any genetic factors that influence IGFBP3 protein levels?

Yes, genetic variations can affect IGFBP3 expression, potentially leading to differences in protein levels among individuals.

How does IGFBP3 protein influence bone health?

IGFBP3 is involved in bone metabolism and is associated with bone mineral density. Low levels of IGFBP3 have been linked to decreased bone density and increased risk of fractures.

Are there any side effects associated with therapies targeting IGFBP3?

The potential side effects of therapies targeting IGFBP3 are still being studied, and it varies depending on the specific treatment approach being used.

How do lifestyle factors such as diet and exercise influence IGFBP3 levels?

Healthy lifestyle choices, including a balanced diet and regular exercise, can positively influence IGFBP3 levels, promoting overall health and reducing the risk of related diseases.

What are the implications of IGFBP3 deficiency in children's growth and development?

IGFBP3 deficiency can impact children's growth and development, potentially leading to short stature and delayed puberty, as IGFBP3 is a key regulator of growth hormone actions.