Recombinant Human IL17 protein(Gly24-Ala155), His-tagged
Cat.No. : | IL17A-445H |
Product Overview : | Recombinant Human IL17A (Q16552, with mutation Thr 26 Ala) (Gly24-Ala155) was expressed in Yeast with a polyhistidine tag at the N-terminus. |
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Source : | Yeast |
Species : | Human |
Tag : | N-His |
Protein length : | Gly24-Ala155 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized Human IL17 His at 2 μg/ml (100 μl/well) can bind Human IL17RA His & hFc, the EC50 of Human IL17RA His & hFc is 30-180 ng/mL. 2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The EC50 for this effect is typically 5-25 ng/ml. |
Molecular Mass : | The recombinant human human IL17A consists of 152 amino acids and predicts a molecular mass of 17.3 KDa. It migrates as an approximately 17 KDa band in SDS-PAGE under reducing conditions. |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
◆ Recombinant Protein | ||
IL17A-7334H | Recombinant Human IL17 protein(Gly24-Ala155(T26A)), His-tagged | +Inquiry |
IL17A-7335H | Recombinant Human IL17 protein(Ile20-Ala155) | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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