Recombinant Human ILK Protein (1-228 aa), His-tagged

• Cat.No.: ILK-1179H
• Product Overview: Recombinant Human ILK Protein (1-228 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Partial.

Specification

• Description: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 30.0 kDa
• Protein length: 1-228 aa
• AA Sequence: MDDIFTQCREGNAVAVRLWLDNTEN DLNQGDDHGFSPLHWACREGRSAVV EMLIMRGARINVMNRGDDTPLHLAA SHGHRDIVQKLLQYKADINAVNEHG NVPLHYACFWGQDQVAEDLVANGAL VSICNKYGEMPVDKAKAPLRELLRE RAEKMGQNLNRIPYKDTFWKGTTRT RPRNGTLNKHSGIDFKQLNFLTKLN ENHSGELWKGRWQGNDIVVKVLKVR DWS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: ILK integrin-linked kinase [ Homo sapiens ]
• Official Symbol: ILK
• Synonyms: ILK; ILK-1; p59ILK; P59; ILK-2; DKFZp686F1765
• Gene ID: 3611
• mRNA Refseq: NM_001014794
• Protein Refseq: NP_001014794
• MIM: 602366
• UniProt ID: Q13418