Recombinant Human ILKAP protein(Met1-His392), His-tagged

• Cat.No.: ILKAP-1126H
• Product Overview: Recombinant Human ILKAP (Q9H0C8) (Met 1-His 392) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-His392
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, 20%Glycerol, 1mM DTT, pH 7. 5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human ILKAP comprises 403 amino acids with a predicted molecular mass of 44.3 kDa. The apparent molecular mass of rh ILKAP is approximately 46 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ILKAP integrin-linked kinase-associated serine/threonine phosphatase [ Homo sapiens ]
• Official Symbol: ILKAP
• Synonyms: ILKAP; integrin-linked kinase-associated serine/threonine phosphatase; integrin linked kinase associated serine/threonine phosphatase 2C; integrin-linked kinase-associated serine/threonine phosphatase 2C; DKFZP434J2031; FLJ10181; protein phosphatase 2c, delta isozyme; integrin-linked kinase associated phosphatase; ILKAP2; ILKAP3; PP2C-DELTA; MGC4846; DKFZp434J2031
• Gene ID: 80895
• mRNA Refseq: NM_030768
• Protein Refseq: NP_110395
• UniProt ID: Q9H0C8

Reviews

11/04/2022

No doping impurities, the experimental results are very good, will recommend to other students.

05/26/2022

The sensitivity is very high, the experimental results are very good, and the unnecessary trouble is reduced.

09/30/2021

Not easy to inactivate, tried a variety of experimental conditions, still active.

Q&As

What is the function of ILKAP? What is the localization inside the cell?

ILKAP is a serine/threonine protein phosphatase that is intracellular localized in the cytoplasm and cytoskeleton and interacts with Integrin-linked kinase (ILK).

Which signaling pathways are involved in ILKAP?

It has been shown that ILKAP may interact with NF-κB and JAK/STAT signaling pathways, but further studies are needed to clarify the specific role and mechanism.

How does ILKAP play a role in immune regulation?

ILKAP may be involved in the process of immune regulation and affect the activity and response of immune cells, but the specific role needs to be further studied.

Is ILKAP expression level correlated with tumor prognosis?

Some studies have shown that ILKAP expression level decreased in some tumors may be associated with poor prognosis of tumour, but the specific mechanism still need more study.

What is the role of ILKAP in apoptosis?

Several studies have suggested that ILKAP may play a role in apoptosis, thereby affecting cell survival and proliferation.

How do ILKAP and ILK interact with each other?

The interaction between ILKAP and ILK, and may be involved in cell adhesion, movement and biological processes such as signal transmission.