Recombinant Human immature colon carcinoma transcript 1, His-tagged

• Cat.No.: ICT1-395H
• Product Overview: ICT1, 30-206aa, Human, His tag, E.coli

Specification

• Description: Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. It may be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Recombinant human ICT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 22.8 kDa (200aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLHKQKDG TEFKSIYSLD KLYPESQGSD TAWRVPNGAK QADSDIPLDR LTISYCRSSG PGGQNVNKVN SKAEVRFHLA TAEWIAEPVR QKIAITHKNK INRLGELILT SESSRYQFRN LADCLQKIRD MITEASQTPK EPTKEDVKLH RIRIENMNRE RLRQKRIHSA VKTSRRVDMD
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ICT1 immature colon carcinoma transcript 1 [ Homo sapiens ]
• Official Symbol: ICT1
• Synonyms: ICT1; immature colon carcinoma transcript 1; peptidyl-tRNA hydrolase ICT1, mitochondrial; DS 1; digestion substraction 1; immature colon carcinoma transcript 1 protein; DS1; DS-1
• Gene ID: 3396
• mRNA Refseq: NM_001545
• Protein Refseq: NP_001536
• MIM: 603000
• UniProt ID: Q14197
• Chromosome Location: 17q25
• Function: aminoacyl-tRNA hydrolase activity; hydrolase activity; translation release factor activity, codon nonspecific