Recombinant Human ING3 293 Cell Lysate

• Cat.No.: ING3-5207HCL

Specification

• Description: Antigen standard for inhibitor of growth family, member 3 (ING3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ING3 inhibitor of growth family, member 3 [ Homo sapiens ]
• Official Symbol: ING3
• Synonyms: ING3; inhibitor of growth family, member 3; inhibitor of growth protein 3; Eaf4; FLJ20089; MEAF4; p47ING3; ING2
• Gene ID: 54556
• mRNA Refseq: NM_019071
• Protein Refseq: NP_061944
• MIM: 607493
• UniProt ID: Q9NXR8
• Chromosome Location: 7q31
• Function: contributes_to histone acetyltransferase activity; metal ion binding; methylated histone residue binding; zinc ion binding

Reviews

05/15/2022

 The product’s unique attributes propelled our research to innovative heights.

09/08/2020

Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.

09/16/2018

 The product consistently met our expectations, reinforcing the credibility of our findings.

Q&As

What specific modification does ING3 recognize on histones, and where is this recognition mediated?

ING3 recognizes histone H3 trimethylated at Lysine 4 (H3K4me3) through the Plant HomeoDomain (PHD) present at its C-terminal region.

What structural similarity was observed between ING3’s PHD and that of its homologs?

The crystal structure of ING3’s PHD showed similarity to the PHD domains of its four homologs, suggesting a common structural motif among them.

What enzymatic activity does ING3 facilitate regarding histones H2A and H4?

ING3 facilitates acetylation of histones H2A and H4 by interacting with the NuA4-Tip60 MYST histone acetyltransferase complex.

How does the PHD of ING3 interact with histone H3K4me3?

The PHD of ING3 binds histone H3K4me3 with low-micromolar affinity, while its affinity for non-methylated histone is reduced by 54-fold.

What insights do these structural features provide regarding ING3 mutations in tumors?

The structural features explain the possible deleterious effects of ING3 mutations detected in tumors, which may disrupt its histone recognition and functional roles.

How has ING3 been characterized in relation to cancer?

ING3 has been proposed to be an oncoprotein, suggesting its potential involvement in tumorigenesis.

What structural features were observed in the crystal structure of ING3’s N-terminal domain?

The crystal structure of the N-terminal domain of ING3 revealed that it forms homodimers with an antiparallel coiled-coil fold.