Recombinant Human INPP5F Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: INPP5F-5409H
• Product Overview: INPP5F MS Standard C13 and N15-labeled recombinant protein (NP_055752) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 128.2 kDa
• AA Sequence: MELFQAKDHYILQQGERALWCSRRD GGLQLRPATDLLLAWNPICLGLVEG VIGKIQLHSDLPWWLILIRQKALVG KLPGDHEVCKVTKIAVLSLSEMEPQ DLELELCKKHHFGINKPEKIIPSPD DSKFLLKTFTHIKSNVSAPNKKKVK ESKEKEKLERRLLEELLKMFMDSES FYYSLTYDLTNSVQRQSTGERDGRP LWQKVDDRFFWNKYMIQDLTEIGTP DVDFWIIPMIQGFVQIEELVVNYTE SSDDEKSSPETPPQESTCVDDIHPR FLVALISRRSRHRAGMRYKRRGVDK NGNVANYVETEQLIHVHNHTLSFVQ TRGSVPVFWSQVGYRYNPRPRLDRS EKETVAYFCAHFEEQLNIYKKQVII NLVDQAGREKVIGDAYLKQVLLFNN SHLTYVSFDFHEHCRGMKFENVQTL TDAIYDIILDMKWCWVDEAGVICKQ EGIFRVNCMDCLDRTNVVQAAIARV VMEQQLKKLGVMPPEQPLPVKCNRI YQIMWANNGDSISRQYAGTAALKGD FTRTGERKLAGVMKDGVNSANRYYL NRFKDAYRQAVIDLMQGIPVTEDLY SIFTKEKEHEALHKENQRSHQELIS QLLQSYMKLLLPDDEKFHGGWALID CDPSLIDATHRDVDVLLLLSNSAYY VAYYDDEVDKVNQYQRLSLENLEKI EIGPEPTLFGKPKFSCMRLHYRYKE ASGYFHTLRAVMRNPEEDGKDTLQC IAEMLQITKQAMGSDLPIIEKKLER KSSKPHEDIIGIRSQNQGSLAQGKN FLMSKFSSLNQKVKQTKSNVNIGNL RKLGNFTKPEMKVNFLKPNLKVNLW KSDSSLETMENTGVMDKVQAESDGD MSSDNDSYHSDEFLTNSKSDEDRQL ANSLESVGPIDYVLPSCGIIASAPR LGSRSQSLSSTDSSVHAPSEITVAH GSGLGKGQESPLKKSPSAGDVHILT GFAKPMDIYCHRFVQDAQNKVTHLS ETRSVSQQASQERNQMTNQVSDETQ SESTEQTPSRPSQLDVSLSATGPQF LSVEPAHSVASQKTPTSASSMLELE TGLHVTPSPSESSSSRAVSPFAKIR SSMVQVASITQAGLTHGINFAVSKV QKSPPEPEIINQVQQNELKKMFIQC QTRIIQITRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: INPP5F inositol polyphosphate-5-phosphatase F [ Homo sapiens (human) ]
• Official Symbol: INPP5F
• Synonyms: INPP5F; inositol polyphosphate-5-phosphatase F; phosphatidylinositide phosphatase SAC2; hSac2; KIAA0966; SAC2; Sac domain-containing inositol phosphatase 2; sac domain-containing phosphoinositide 5-phosphatase 2; hSAC2; MSTP007; MSTPO47; FLJ13081; MGC59773; MGC131851
• Gene ID: 22876
• mRNA Refseq: NM_014937
• Protein Refseq: NP_055752
• MIM: 609389
• UniProt ID: Q9Y2H2

Reviews

05/18/2020

Its speed in modulating signaling pathways allowed us to obtain results quickly, saving valuable time.

10/25/2017

The product's biocompatibility with various cell types and organisms made it versatile for a wide range of applications.

02/21/2017

The product's stability over extended periods was a significant advantage for our long-term research projects.

Q&As

How does INPP5F affect HCC cell proliferation?

Overexpression of INPP5F promotes HCC cell proliferation, while its knockdown suppresses tumor growth.

How does INPP5F influence lactate production and glucose consumption in HCC cells?

INPP5F enhances lactate production and glucose consumption in HCC cells.

What is the role of INPP5F in hepatocellular carcinoma (HCC)?

INPP5F plays a role in HCC and is associated with tumor growth and progression.

What is the impact of INPP5F's subcellular location in HCC?

In HCC, INPP5F is commonly located in the cytoplasm, whereas it is nuclear-located in cells of adjacent non-tumor tissues.

What signaling pathway is activated by INPP5F in HCC?

INPP5F activates the Notch signaling pathway in HCC.

What is the expression pattern of INPP5F in HCC tissues?

INPP5F is highly expressed in HCC tissues and is associated with poor prognosis in HCC patients.

What genes and processes are regulated by INPP5F in HCC?

INPP5F regulates cell cycle-related genes (c-MYC and cyclin E1) and promotes aerobic glycolysis-related genes.