Recombinant Human KDM4D Protein, Strep II-tagged

• Cat.No.: KDM4D-123H
• Product Overview: Active recombinant N-terminal Strep II-tagged human KDM4D protein was expressed in E. coli.

Specification

• Description: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
• Source: E. coli
• Species: Human
• Tag: Strep II
• Molecular Mass: 42.6 kDa
• Purity: ≥70% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of NADH per minute at 37 centigrade in 50 mM HEPES buffer, pH 7.4, containing 50 mM sodium chloride, 1 mM ascorbic acid, 50 μM ferrous ammonium sulfate, 1 mM NAD+, 1 mM α-ketoglutarate, 0.1 μM FDH, and 125 μM H3K9me3 peptide.
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: 20 mM HEPES, pH 7.4, and 150 mM sodium chloride

Gene Information

• Gene Name: KDM4D lysine demethylase 4D [ Homo sapiens (human) ]
• Official Symbol: KDM4D
• Synonyms: KDM4D; lysine demethylase 4D; JMJD2D; lysine-specific demethylase 4D; [histone H3]-trimethyl-L-lysine(9) demethylase 4D; jmjC domain-containing histone demethylation protein 3D; jumonji domain containing 2D; jumonji domain-containing protein 2D; lysine (K)-specific demethylase 4D; EC 1.14.11.66
• Gene ID: 55693
• mRNA Refseq: NM_018039
• Protein Refseq: NP_060509
• MIM: 609766
• UniProt ID: Q6B0I6