Recombinant Human LGR5 HEK293T cell lysate

• Cat.No.: LGR5-12HCL
• Product Overview: Human LGR5 was derived from Human HEK293T cell line.

Specification

• Description: The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293T
• Species: Human
• Tag: C-terminal Myc/DDK
• Form: The cell lysate is provided in RIPA buffer.
• Molecular Mass: 99.8 kDa
• Usage: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: LGR5 leucine-rich repeat containing G protein-coupled receptor 5 [ Homo sapiens ]
• Official Symbol: LGR5
• Synonyms: LGR5; leucine-rich repeat containing G protein-coupled receptor 5; G protein coupled receptor 49 , GPR49, GPR67, leucine rich repeat containing G protein coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; FEX; HG38; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; orphan G protein-coupled receptor HG38; leucine-rich repeat-containing G protein-coupled receptor 5; GPR49; GPR67; GRP49; MGC117008
• Gene ID: 8549
• mRNA Refseq: NM_003667
• Protein Refseq: NP_003658
• MIM: 606667
• UniProt ID: O75473
• Chromosome Location: 12q22-q23
• Function: G-protein coupled receptor activity; protein-hormone receptor activity; receptor activity; signal transducer activity

Reviews

09/06/2021

Critical for decision-making, enhances our research quality.

01/29/2020

Enhances research credibility, critical for research integrity.

06/08/2019

Efficiency and precision, integral to our research.

Q&As

What is the primary role of LGR5 in stem cell biology?

LGR5, a G protein-coupled receptor, is crucial for the maintenance and proliferation of adult stem cells.

How do genetic variations in LGR5 affect tissue homeostasis?

Genetic mutations in LGR5 can disrupt normal stem cell function and tissue maintenance.

How does LGR5 contribute to tissue regeneration?

It plays a key role in promoting tissue regeneration by signaling stem cells to divide and differentiate.

What role does LGR5 play in Wnt signaling?

LGR5 acts as a co-receptor in the Wnt signaling pathway, essential for regulating stem cell growth.

What is the impact of altered LGR5 activity on cancer development?

Altered LGR5 activity is associated with the growth of certain cancers, particularly colorectal cancer.

How does LGR5 interact with other components in the Wnt pathway?

LGR5 works alongside Wnt ligands and receptors, enhancing Wnt-mediated signaling in stem cells.

What potential therapeutic applications arise from targeting LGR5 in regenerative medicine and oncology?

Targeting LGR5 offers potential for both regenerating tissues in degenerative diseases and targeting cancer stem cells.