Description : |
The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
His |
Form : |
Filtered (0.4 μm) and lyophilized in phosphate buffered saline pH=7.5 (PBS), 1% (w/v) Sucrose, 4% (w/v) Mannitol |
Molecular Mass : |
55.8 kDa (calculated) |
Protein length : |
Ser21–Pro500 |
AA Sequence : |
ASSPVPFGPEGRLEDKLHKPKATQT EVKPSVRFNLRTSKDPEHEGCYLSV GHSQPLEDCSFNMTAKTFFIIHGWT MSGIFENWLHKLVSALHTREKDANV VVVDWLPLAHQLYTDAVNNTRVVGH SIARMLDWLQEKDDFSLGNVHLIGY SLGAHVAGYAGNFVKGTVGRITGLD PAGPMFEGADIHKRLSPDDADFVDV LHTYTRSFGLSIGIQMPVGHIDIYP NGGDFQPGCGLNDVLGSIAYGTITE VVKCEHERAVHLFVDSLVNQDKPSF AFQCTDSNRFKKGICLSCRKNRCNS IGYNAKKMRNKRNSKMYLKTRAGMP FRVYHYQMKIHVFSYKNMGEIEPTF YVTLYGTNADSQTLPLEIVERIEQN ATNTFLVYTEEDLGDLLKIQLTWEG ASQSWYNLWKEFRSYLSQPRNPGRE LNIRRIRVKSGETQRKLTFCTEDPE NTSISPGRELWFRKCRDGWRMKNET SPTVELPKLHHHHHH |
Endotoxin : |
< 1.0 EU/ug |
Purity : |
Purity as determined by densitometric image analysis: > 85 % |
Applications : |
ELISA, Cell culture and/or animal studies |
Storage : |
Store the lyophilized protein at –80 centigrade. Lyophilized protein remains stable until the expiry date when stored at –80 centigrade. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 centigrade for long term storage. Reconstituted protein can be stored at 4 centigrade for a week. |
Reconstitution : |
Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture. |