Recombinant Human LRRK2 HEK293T cell lysate

• Cat.No.: LRRK2-35HCL
• Product Overview: Human LRRK2 was derived from Human HEK293T cell line.

Specification

• Description: This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.
• Source: HEK293
• Species: Human
• Tag: C-terminal Myc/DDK
• Form: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer(25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4).
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer.
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT).
• Molecular Mass: 285.9 kDa
• Storage: Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Shipping: The lysate is shipped with dry ice

Gene Information

• Gene Name: LRRK2 leucine-rich repeat kinase 2 [ Homo sapiens ]
• Official Symbol: LRRK2
• Synonyms: LRRK2; leucine-rich repeat kinase 2; PARK8, Parkinson disease (autosomal dominant) 8; leucine-rich repeat serine/threonine-protein kinase 2; DKFZp434H2111; FLJ45829; RIPK7; ROCO2; augmented in rheumatoid arthritis 17; PARK8; AURA17; DARDARIN
• Gene ID: 120892
• mRNA Refseq: NM_198578
• Protein Refseq: NP_940980
• MIM: 609007
• UniProt ID: Q5S007