Recombinant Human MAPK1, His-tagged

• Cat.No.: MAPK1-28700TH
• Product Overview: Recombinant human MAPK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.
• Molecular Mass: 43.5 kDa (380aa), confirmed by MALDI-TOF.
• Protein length: 1-360aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLREIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTTCDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQEDLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPKEKLKELIFEE TARFQPGYRS
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MAPK1 mitogen-activated protein kinase 1 [ Homo sapiens ]
• Official Symbol: MAPK1
• Synonyms: MAPK1; mitogen-activated protein kinase 1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; MAP kinase 2; Mitogen-activated protein kinase 2; Extracellular signal-regulated kinase 2; OTTHUMP00000174492; MAP kinase 2; protein tyrosine kinase ERK2
• Gene ID: 5594
• mRNA Refseq: NM_138957
• Protein Refseq: NP_620407
• MIM: 176948
• UniProt ID: P28482
• Chromosome Location: 22q11.21
• Pathway: AGE/RAGE pathway; ARMS-mediated activation; Acute myeloid leukemia
• Function: RNA polymerase II carboxy-terminal domain kinase activity; mitogen-activated protein kinase kinase kinase binding; phosphatase binding

Reviews

03/24/2022

Whether it be troubleshooting issues, offering experimental guidance, or addressing any concerns, the manufacturer's technical support ensures that researchers have a reliable and knowledgeable resource to rely on.

03/26/2021

In addition to its scientific merits, the manufacturer of MAPK1 protein stands out for its outstanding technical support.

03/11/2020

The company provides a dedicated team of experts who are readily available to assist researchers throughout their experimental journey.

Q&As

Can MAPK1 be used as a biomarker for cancer diagnosis?

MAPK1 expression and activity levels can be used as biomarkers to assess cancer risk and prognosis.

How can MAPK1 be targeted in drug development for non-cancer conditions?

MAPK1 inhibitors are being explored for potential use in non-cancer conditions, such as heart disease and neurodegenerative disorders.

In what other clinical conditions is MAPK1 relevant?

MAPK1 is also implicated in various other diseases, including cardiovascular diseases and neurological disorders.

Are there targeted therapies that inhibit MAPK1 for cancer treatment?

Yes, there are targeted therapies that aim to inhibit MAPK1, especially in cases where MAPK1 is overactive in cancer cells.

Can MAPK1 be used for personalized medicine approaches?

Yes, by assessing the MAPK1 status in a patient's cells, healthcare providers can develop personalized treatment plans.