Recombinant Human MAPKAP1 Protein (2-522 aa), His-SUMO-tagged

• Cat.No.: MAPKAP1-1797H
• Product Overview: Recombinant Human MAPKAP1 Protein (2-522 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Full Length of Mature Protein.

Specification

• Description: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 75.0 kDa
• Protein length: 2-522 aa
• AA Sequence: AFLDNPTIILAHIRQSHVTSDDTGM CEMVLIDHDVDLEKIHPPSMPGDSG SEIQGSNGETQGYVYAQSVDITSSW DFGIRRRSNTAQRLERLRKERQNQI KCKNIQWKERNSKQSAQELKSLFEK KSLKEKPPISGKQSILSVRLEQCPL QLNNPFNEYSKFDGKGHVGTTATKK IDVYLPLHSSQDRLLPMTVVTMASA RVQDLIGLICWQYTSEGREPKLNDN VSAYCLHIAEDDGEVDTDFPPLDSN EPIHKFGFSTLALVEKYSSPGLTSK ESLFVRINAAHGFSLIQVDNTKVTM KEILLKAVKRRKGSQKVSGPQYRLE KQSEPNVAVDLDSTLESQSAWEFCL VRENSSRADGVFEEDSQIDIATVQD MLSSHHYKSFKVSMIHRLRFTTDVQ LGISGDKVEIDPVTNQKASTKFWIK QKPISIDSDLLCACDLAEEKSPSHA IFKLTYLSNHDYKHLYFESDAATVN EIVLKVNYILESRASTARADYFAQK QRKLNRRTSFSFQKEKKSGQQ
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: MAPKAP1 mitogen-activated protein kinase associated protein 1 [ Homo sapiens ]
• Official Symbol: MAPKAP1
• Synonyms: MAPKAP1; MGC2745; MIP1; SIN1; mSIN1; TORC2 subunit MAPKAP1; JC310; SIN1b; SIN1g
• Gene ID: 79109
• mRNA Refseq: NM_001006617
• Protein Refseq: NP_001006618
• MIM: 610558
• UniProt ID: Q9BPZ7