Recombinant Human MBTPS1 Protein (218-414 aa), His-SUMO-tagged

• Cat.No.: MBTPS1-2058H
• Product Overview: Recombinant Human MBTPS1 Protein (218-414 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.

Specification

• Description: Serine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu (SERBP-2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 37.5 kDa
• Protein length: 218-414 aa
• AA Sequence: DTGLSEKHPHFKNVKERTNWTNERT LDDGLGHGTFVAGVIASMRECQGFA PDAELHIFRVFTNNQVSYTSWFLDA FNYAILKKIDVLNLSIGGPDFMDHP FVDKVWELTANNVIMVSAIGNDGPL YGTLNNPADQMDVIGVGGIDFEDNI ARFSSRGMTTWELPGGYGRMKPDIV TYGAGVRGSGVKGGCRALSGTS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: MBTPS1 membrane-bound transcription factor peptidase, site 1 [ Homo sapiens ]
• Official Symbol: MBTPS1
• Synonyms: MBTPS1; KIAA0091; PCSK8; S1P; SKI 1; site-1 protease; endopeptidase S1P; SKI-1; MGC138711; MGC138712
• Gene ID: 8720
• mRNA Refseq: NM_003791
• Protein Refseq: NP_003782
• MIM: 603355
• UniProt ID: Q14703