Recombinant Human MCM7 Protein, Myc/DDK-tagged

• Cat.No.: MCM7-01H
• Product Overview: Recombinant protein of human minichromosome maintenance complex component 7 (MCM7), transcript variant 1 with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Description: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 81.1 kDa
• AA Sequence: MALKDYALEKEKVKKFLQEFYQDDE LGKKQFKYGNQLVRLAHREQVALYV DLDDVAEDDPELVDSICENARRYAK LFADAVQELLPQYKEREVVNKDVLD VYIEHRLMMEQRSRDPGMVRSPQNQ YPAELMRRFELYFQGPSSNKPRVIR EVRADSVGKLVTVRGIVTRVSEVKP KMVVATYTCDQCGAETYQPIQSPTF MPLIMCPSQECQTNRSGGRLYLQTR GSRFIKFQEMKMQEHSDQVPVGNIP RSITVLVEGENTRIAQPGDHVSVTG IFLPILRTGFRQVVQGLLSETYLEA HRIVKMNKSEDDESGAGELTREELR QIAEEDFYEKLAASIAPEIYGHEDV KKALLLLLVGGVDQSPRGMKIRGNI NICLMGDPGVAKSQLLSYIDRLAPR SQYTTGRGSSGVGLTAAVLRDSVSG ELTLEGGALVLADQGVCCIDEFDKM AEADRTAIHEVMEQQTISIAKAGIL TTLNARCSILAAANPAYGRYNPRRS LEQNIQLPAALLSRFDLLWLIQDRP DRDNDLRLAQHITYVHQHSRQPPSQ FEPLDMKLMRRYIAMCREKQPMVPE SLADYITAAYVEMRREAWASKDATY TSARTLLAILRLSTALARLRMVDVV EKEDVNEAIRLMEMSKDSLLGDKGQ TARTQRPADVIFATVRELVSGGRSV RFSEAEQRCVSRGFTPAQFQAALDE YEELNVWQVNASRTRITFV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: MCM7 minichromosome maintenance complex component 7 [ Homo sapiens (human) ]
• Official Symbol: MCM7 minichromosome maintenance complex component 7 [ Homo sapiens (human) ]
• Synonyms: MCM7; minichromosome maintenance complex component 7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3; DNA replication licensing factor MCM7; CDC47 homolog; homolog of S. cerevisiae Cdc47; minichromosome maintenance deficient 7; protein phosphatase 1, regulatory subunit 104; EC 3.6.4.12
• Gene ID: 4176
• mRNA Refseq: NM_005916
• Protein Refseq: NP_005907
• MIM: 600592
• UniProt ID: P33993