Recombinant Human METAP1D Protein, N-Met,C-His-tagged

• Cat.No.: METAP1D-14H
• Product Overview: Recombinant Protein of Human METAP1D, with N-terminal Met and C-terminal His tag, expressed in Sf21.

Specification

• Description: The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18).
• Source: Insect cells
• Species: Human
• Tag: N-Met,C-His
• Protein length: Arg44-Ala335
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC. The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26. The specific activity is >30 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 34 kDa
  SDS-PAGE: 38 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: METAP1D methionyl aminopeptidase type 1D, mitochondrial [ Homo sapiens (human) ]
• Official Symbol: METAP1D
• Synonyms: EC 3.4.11.18; MAP1D; MAP1DCDS of metAP-3 within PCR fragment; MetAP1D; Metap1l; Methionine Aminopeptidase 1D; methionine aminopeptidase 1D, mitochondrial; methionyl aminopeptidase type 1D (mitochondrial); Methionyl aminopeptidase type 1D, mitochondrial; MAP 1D; Metap1l; MetAP 1D
• Gene ID: 254042
• mRNA Refseq: NM_199227.3
• Protein Refseq: NP_954697.1
• MIM: 610267
• UniProt ID: Q6UB28