Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase 5, GST-tagged

• Cat.No.: MAP3K5-1260H
• Product Overview: RecombinantHuman MAP3K5 with N-terminal GST tag. MAP3K5 is a serine/threonine kinasethat is abundantly expressed in human heart and pancreas.

Specification

• Cat. No.: MAP3K5-1260H
• Description: The MAP3K5protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro,and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase(SAPK) during transient expression in COS and 293 cells; MAP3K5 does notactivate MAPK/ERK.
• Source: Insect cells
• Species: Human
• Form: Liquid in50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
• Molecular Weight: 182 kDa
• Specific Activity: 64 nmoleof phosphate transferred to myelin basic protein (MBP) per minute per mg of totalprotein at 30°C. Activity determined at a final protein concentration of 8.33μg/ml.
• Concentration: 0.25mg/ml
• Storage: Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: MAP3K5
• Function: MAP kinase kinasekinase activity; metal ion binding; protein kinase activity; nucleotidebinding; protein serine/tyreonine kinase activity; protein phosphatase binding;protein homodimerization activity; protein binding; magnesium ion binding; cysteine-typeendopeptidase activator activity involved in apoptotic process

Gene Information

• Gene Name: MAP3K5 mitogen-activated proteinkinase kinase kinase 5 [ Homo sapiens ]
• Synonyms: MAP3K5; mitogen-activatedprotein kinase kinase kinase 5; MEKK5; MAPKKK5; ASK1; apoptosissignal regulating kinase 1; OTTHUMP00000017275; EC 2.7.11.25; ASK-1; MEKkinase 5; MAPK/ERK kinase kinase 5; MAP/ERK kinase kinase 5; apoptosis signal-regulatingkinase 1
• Gene ID: 4217
• mRNA Refseq: NM_005923
• Protein Refseq: NP_005914
• MIM: 602448
• UniProt ID: Q99683
• Chromosome Location: 6q22.33
• Pathway: p38 MAPK signalingpathway; Protein processing in endoplasmic reticulum; Class I PI3K signalingevents mediated by Akt; Amyotrophic lateral sclerosis (ALS); HTLV-Iinfection;IL1-mediatedsignaling events; Neurotrophin signaling pathway; InsulinSignaling; TNFreceptor signaling pathway; MAPK Cascade; MAPK signaling pathway; TNF-alpha/NF-kBSignaling Pathway; Trk receptor signaling mediated by the MAPK pathway

Reviews

06/30/2020

The MAP3K5 protein has proven to be invaluable in protein electron microscopy structure analysis.

07/24/2018

Its ability to provide accurate and reliable results in this particular application makes it a preferred choice for researchers studying antigen-antibody interactions and immune responses.

12/01/2016

The MAP3K5 protein's sensitivity and specificity ensure precise measurements, contributing to the overall success of ELISA experiments.

Q&As

What other diseases or conditions involve MAP3K5?

MAP3K5 has been implicated in various diseases, including neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions.

How is MAP3K5 protein implicated in cancer?

Aberrant regulation of MAP3K5 has been associated with cancer development, as it can influence cell survival and death pathways.

Can MAP3K5 be used as a biomarker for certain diseases?

MAP3K5 expression levels and activity may serve as biomarkers for specific diseases, providing valuable information for diagnosis and prognosis.

Are there any clinical applications of MAP3K5 in cancer treatment?

Research suggests that targeting MAP3K5 could have therapeutic implications in cancer treatment, potentially through the modulation of apoptosis in cancer cells.

Are there ongoing clinical trials involving MAP3K5 inhibitors?

Investigational studies and clinical trials are underway to explore the efficacy of MAP3K5 inhibitors in different disease contexts, including cancer.