Recombinant Human MLH1 protein, His-tagged

• Cat.No.: MLH1-1291H
• Product Overview: Recombinant Human MLH1 protein(Ser486~Lys751), fused with N-terminal His Tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 35kDa
• Protein Length: Ser486~Lys751
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) [ Homo sapiens ]
• Official Symbol: MLH1
• Synonyms: MLH1; mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); COCA2, mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2); DNA mismatch repair protein Mlh1; FCC2; HNPCC; HNPCC2; COCA2; hMLH1; MGC5172
• Gene ID: 4292
• mRNA Refseq: NM_000249
• Protein Refseq: NP_000240
• MIM: 120436
• UniProt ID: P40692

Reviews

11/23/2022

Timely protein expression optimization, greatly boosts our research.

04/22/2022

Accurate protein structural analysis, a cornerstone of our investigations.

10/01/2020

Precise antibody validation, enhances our immunoprecipitation accuracy.

Q&As

What is the impact of germline mutations in MLH1 on Lynch syndrome and associated cancer risks?

Germline mutations in MLH1 are linked to Lynch syndrome, increasing the risk of various cancers.

What role does MLH1 play in the prevention of colorectal and other hereditary cancers?

MLH1 helps prevent the development of colorectal and other hereditary cancers by maintaining DNA integrity.

How do alterations in MLH1 expression or function affect chemotherapy efficacy in cancer treatment?

Changes in MLH1 function can influence the effectiveness of certain chemotherapies in treating cancer.

How does MLH1 influence the cellular response to DNA damage and the maintenance of replication fidelity?

MLH1 plays a role in the cellular response to DNA damage, maintaining replication accuracy.

How does MLH1 deficiency lead to microsatellite instability in cancer cells?

MLH1 deficiency results in microsatellite instability, a hallmark of certain types of cancer.

How does MLH1 (MutL Homolog 1) contribute to DNA mismatch repair and genomic stability?

MLH1 is essential for DNA mismatch repair, ensuring genomic stability by correcting replication errors.

What are the mechanisms by which MLH1 modulates apoptosis in response to DNA damage?

MLH1 modulates apoptosis in cells with DNA damage, preventing the proliferation of damaged cells.