Recombinant Human MLH1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MLH1-2320H
• Product Overview: MLH1 MS Standard C13 and N15-labeled recombinant protein (NP_000240) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 84.6 kDa
• AA Sequence: MSFVAGVIRRLDETVVNRIAAGEVI QRPANAIKEMIENCLDAKSTSIQVI VKEGGLKLIQIQDNGTGIRKEDLDI VCERFTTSKLQSFEDLASISTYGFR GEALASISHVAHVTITTKTADGKCA YRASYSDGKLKAPPKPCAGNQGTQI TVEDLFYNIATRRKALKNPSEEYGK ILEVVGRYSVHNAGISFSVKKQGET VADVRTLPNASTVDNIRSIFGNAVS RELIEIGCEDKTLAFKMNGYISNAN YSVKKCIFLLFINHRLVESTSLRKA IETVYAAYLPKNTHPFLYLSLEISP QNVDVNVHPTKHEVHFLHEESILER VQQHIESKLLGSNSSRMYFTQTLLP GLAGPSGEMVKSTTSLTSSSTSGSS DKVYAHQMVRTDSREQKLDAFLQPL SKPLSSQPQAIVTEDKTDISSGRAR QQDEEMLELPAPAEVAAKNQSLEGD TTKGTSEMSEKRGPTSSNPRKRHRE DSDVEMVEDDSRKEMTAACTPRRRI INLTSVLSLQEEINEQGHEVLREML HNHSFVGCVNPQWALAQHQTKLYLL NTTKLSEELFYQILIYDFANFGVLR LSEPAPLFDLAMLALDSPESGWTEE DGPKEGLAEYIVEFLKKKAEMLADY FSLEIDEEGNLIGLPLLIDNYVPPL EGLPIFILRLATEVNWDEEKECFES LSKECAMFYSIRKQYISEESTLSGQ QSEVPGSIPNSWKWTVEHIVYKALR SHILPPKHFTEDGNILQLANLPDLY KVFERCTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MLH1 mutL homolog 1 [ Homo sapiens (human) ]
• Official Symbol: MLH1
• Synonyms: MLH1; mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); COCA2, mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2); DNA mismatch repair protein Mlh1; FCC2; HNPCC; HNPCC2; COCA2; hMLH1; MGC5172
• Gene ID: 4292
• mRNA Refseq: NM_000249
• Protein Refseq: NP_000240
• MIM: 120436
• UniProt ID: P40692

Reviews

11/23/2022

Timely protein expression optimization, greatly boosts our research.

04/22/2022

Accurate protein structural analysis, a cornerstone of our investigations.

10/01/2020

Precise antibody validation, enhances our immunoprecipitation accuracy.

Q&As

What is the impact of germline mutations in MLH1 on Lynch syndrome and associated cancer risks?

Germline mutations in MLH1 are linked to Lynch syndrome, increasing the risk of various cancers.

What role does MLH1 play in the prevention of colorectal and other hereditary cancers?

MLH1 helps prevent the development of colorectal and other hereditary cancers by maintaining DNA integrity.

How do alterations in MLH1 expression or function affect chemotherapy efficacy in cancer treatment?

Changes in MLH1 function can influence the effectiveness of certain chemotherapies in treating cancer.

How does MLH1 influence the cellular response to DNA damage and the maintenance of replication fidelity?

MLH1 plays a role in the cellular response to DNA damage, maintaining replication accuracy.

How does MLH1 deficiency lead to microsatellite instability in cancer cells?

MLH1 deficiency results in microsatellite instability, a hallmark of certain types of cancer.

How does MLH1 (MutL Homolog 1) contribute to DNA mismatch repair and genomic stability?

MLH1 is essential for DNA mismatch repair, ensuring genomic stability by correcting replication errors.

What are the mechanisms by which MLH1 modulates apoptosis in response to DNA damage?

MLH1 modulates apoptosis in cells with DNA damage, preventing the proliferation of damaged cells.