Recombinant Human MYH10 Over-expression Lysate, Myc/DDK-tagged

• Cat.No.: MYH10-01H
• Product Overview: Transient overexpression lysate of myosin, heavy chain 10, non-muscle (MYH10) with a C-Myc/DDK tag was expressed in HEK293T.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg whole HEK293T cell lysate in RIPA buffer
  1 vial of 250 μL 2×SDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Specification

• Description: This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 228.8 kDa
• Storage: Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
• Dilutions: Lysate samples can be diluted with 2×SDS Sample Buffer provided.

Gene Information

• Gene Name: MYH10 myosin heavy chain 10 [ Homo sapiens (human) ]
• Official Symbol: MYH10
• Synonyms: MYH10; myosin heavy chain 10; NMMHCB; NMMHC-IIB; myosin-10; myosin heavy chain, nonmuscle type B; myosin, heavy chain 10, non-muscle; myosin, heavy polypeptide 10, non-muscle; nonmuscle myosin II heavy chain-B; nonmuscle myosin heavy chain IIB
• Gene ID: 4628
• mRNA Refseq: NM_005964
• Protein Refseq: NP_005955
• MIM: 160776
• UniProt ID: P35580