Recombinant Human MYH8 Protein, His-tagged

• Cat.No.: MYH8-2469H
• Product Overview: Recombinant Human MYH8 Protein (Ile817-Glu1081) with a N-His tag was expressed in E. coli.

Specification

• Description: Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.7 kDa
  Accurate Molecular Mass: 38 kDa
• Protein length: Ile817-Glu1081
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MYH8 myosin heavy chain 8 [ Homo sapiens (human) ]
• Official Symbol: MYH8
• Synonyms: MYH8; myosin heavy chain 8; DA7; MyHC-pn; gtMHC-F; MyHC-peri; myosin-8
  fetal-myosin heavy chain
  myHC-perinatal
  myosin heavy chain, skeletal muscle, perinatal
  myosin, heavy chain 8, skeletal muscle, perinatal
  myosin, heavy polypeptide 8, skeletal muscle, perinatal
• Gene ID: 4626
• mRNA Refseq: NM_002472
• Protein Refseq: NP_002463
• MIM: 160741
• UniProt ID: P13535