Recombinant Human NDNF Protein, His-tagged

• Cat.No.: NDNF-2482H
• Product Overview: Recombinant Human NDNF Protein (Gln20-Phe331) with a N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable heparin binding activity. Involved in several processes, including cellular response to hypoxia; negative regulation of apoptotic process; and nitric oxide mediated signal transduction. Located in extracellular space. Implicated in hypogonadotropic hypogonadism.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 39.0 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Gln20-Phe331
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: NDNF neuron derived neurotrophic factor [ Homo sapiens (human) ]
• Official Symbol: NDNF
• Synonyms: NDNF; neuron derived neurotrophic factor; HH25; NORD; C4orf31; protein NDNF
  fibronectin type-III domain-containing protein C4orf31
• Gene ID: 79625
• mRNA Refseq: NM_024574
• Protein Refseq: NP_078850
• MIM: 616506
• UniProt ID: Q8TB73