| Description : |
The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
79.44 kDa |
| AA Sequence : |
MLRIPVRKALVGLSKSPKGCVRTTA TAASNLIEVFVDGQSVMVEPGTTVL QACEKVGMQIPRFCYHERLSVAGNC RMCLVEIEKAPKVVAACAMPVMKGW NILTNSEKSKKAREGVMEFLLANHP LDCPICDQGGECDLQDQSMMFGNDR SRFLEGKRAVEDKNIGPLVKTIMTR CIQCTRCIRFASEIAGVDDLGTTGR GNDMQVGTYIEKMFMSELSGNIIDI CPVGALTSKPYAFTAQPWETRKTES IDVMDAVGSNIVVSTRTGEVMRILP RMHEDINEEWISDKTRFAYDGLKRQ RLTEPMVRNEKGLLTYTSWEDALSR VAGMLQSFQGKDVAAIAGGLVDAEA LVALKDLLNRVDSDTLCTEEVFPTA GAGTDLRSNYLLNTTIAGVEEADVV LLVGTNPRFEAPLFNARIRKSWLHN DLKVALIGSPVDLTYTYDHLGDSPK ILQDIASGSHPFSQVLKEAKKPMVV LGSSALQRNDGAAILAAVSSIAQKI RMTSGVTGDWKVMNILHRIASQVAA LDLGYKPGVEAIRKNPPKVLFLLGA DGGCITRQDLPKDCFIIYQGHHGDV GAPIADVILPGAAYTEKSATYVNTE GRAQQTKVAVTPPGLAREDWKIIRA LSEIAGMTLPYDTLDQVRNRLEEVS PNLVRYDDIEGANYFQQANELSKLV NQQLLADPLVPPQLTIKDFYMTDSI SRASQTMAKCVKAVTEGAQAVEEPS ICTRTRPLEQKLISEEDLAANDILD YKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
