Recombinant Human NECTIN2 protein(Met1-Leu360), hFc&Avi-tagged, Biotinylated
Cat.No. : | NECTIN2-0632H |
Product Overview : | Recombinant Human NECTIN2 (NP_002847.1)(Met1-Leu360) was expressed in HEK293 with a c-terminal AVI tagged Fc region of Human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
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Source : | HEK293 |
Species : | Human |
Tag : | C-hFc&Avi |
Protein length : | Met1-Leu360 |
Conjugation/Label : | Biotin |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized Human PVRIG hFc at 2 μg/mL (100 μL/well) can bind Human Nectin-2 (ECD, hFc & AVI Tag), Biotinylated, the EC50 of Human Nectin-2 (ECD, hFc & AVI Tag), Biotinylated is 10-60 ng/mL. |
Molecular Mass : | The recombinant human NECTIN2 consists of 582 amino acids and predicts a molecular mass of 64.08 kDa. It migrates as an approximately 75.99 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
◆ Recombinant Protein | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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