Recombinant Human NFKBIA protein(Phe2-Leu317), His-tagged

• Cat.No.: NFKBIA-3340H
• Product Overview: Recombinant Human NFKBIA (NP_065390.1) (Phe 2-Leu 317) was expressed in E. coli, fused with a polyhistide tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: Phe2-Leu317
• Form: Lyophilized from sterile 50mM Tris, 0.5M NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human NFKBIA consisting of 323 amino acids and has a calculated molecular mass of 36.4 kDa. It migRates as an approximately 38 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NFKBIA nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha [ Homo sapiens ]
• Official Symbol: NFKBIA
• Synonyms: NFKBIA; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; NFKBI; NF-kappa-B inhibitor alpha; IkappaBalpha; IKBA; MAD 3; ikB-alpha; I-kappa-B-alpha; nuclear factor of kappa light chain gene enhancer in B-cells; major histocompatibility complex enhancer-binding protein MAD3; MAD-3
• Gene ID: 4792
• mRNA Refseq: NM_020529
• Protein Refseq: NP_065390
• MIM: 164008
• UniProt ID: P25963

Reviews

07/04/2022

I am very happy with the results, they performed very well in my experiments.

05/26/2022

It has high sensitivity and strong specificity.

04/07/2022

It is easy to use and requires little preparation before the experiment.

Q&As

What is the interaction between NFKBIA protein and NF-κB?

The interaction between NFKBIA protein and NF-κB can be identified by co-immunoprecipitation, bimolecular fluorescence complementation, etc. In addition, these methods can also be used to study the interaction sites and mechanisms of action between them.

What is the effect of knocking out the NFKBIA gene on cell growth and apoptosis?

Knockdown of the NFKBIA gene leads to increased cell growth arrest and apoptosis, which may be related to the aberrant activation of the NF-κB signaling pathway.

How can I study the effect of NFKBIA protein on the NF-κB signaling pathway?

The effect on the expression level and activity of molecules related to the NF-κB signaling pathway can be observed by overexpressing or knocking down NFKBIA protein. In addition, studies can also be performed with specific knockout or mutant cell lines.

Is it possible to knock out NFKBIA by gene knockout?

Its role in physiological and pathological processes can be studied by specifically knocking out the NFKBIA gene using specific short hairpin RNA or zinc finger nuclease technology.

How does the expression level of NFKBIA protein relate to disease?

Studies have shown that the expression level of NFKBIA protein is associated with the occurrence and progression of a variety of diseases. For example, in some cancers, the expression levels of the NFKBIA protein are reduced or missing, leading to aberrant activation of the NF-κB signaling pathway.

Do NFKBIA proteins regulate the NF-κB signaling pathway?

When cells are stimulated by inflammation, the NFKBIA protein is phosphorylated, causing its dissociation from NF-κB. After dissociation, NF-κB is transferred to the nucleus and activates the transcription of specific genes. In addition, NFKBIA protein can further regulate the activity of NF-κB by interacting with other proteins.