Recombinant Human NGLY1 Protein, Myc/DDK-Tagged

• Cat.No.: NGLY1-1828H
• Product Overview: Recombinant human NGLY1 protein, C-Myc/DDK-tagged, was expressed in HEK293T cell

Specification

• Description: This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene
• Source: HEK293T
• Species: Human
• Tag: C-Myc/DDK
• Cytogenetics: 3p24.2
• Refseq Size: 2534
• Refseq ORF: 1965
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Storage: Store at -80°C.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Molecular Mass: 74.2 kDa
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: NGLY1 N-glycanase 1 [ Homo sapiens (human) ]
• Official Symbol: NGLY1
• Synonyms: CDDG; PNG1; CDG1V; PNG-1; PNGase
• Gene ID: 55768
• mRNA Refseq: NM_001145293.2
• Protein Refseq: NP_060767
• UniProt ID: Q96IV0
• MIM: 610661