Recombinant Human NPL Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NPL-4467H
• Product Overview: NPL MS Standard C13 and N15-labeled recombinant protein (NP_110396) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 35.2 kDa
• AA Sequence: MAFPKKKLQGLVAATITPMTENGEI NFSVIGQYVDYLVKEQGVKNIFVNG TTGEGLSLSVSERRQVAEEWVTKGK DKLDQVIIHVGALSLKESQELAQHA AEIGADGIAVIAPFFLKPWTKDILI NFLKEVAAAAPALPFYYYHIPALTG VKIRAEELLDGILDKIPTFQGLKFS DTDLLDFGQCVDQNRQQQFAFLFGV DEQLLSALVMGATGAVGSTYNYLGK KTNQMLEAFEQKDFSLALNYQFCIQ RFINFVVKLGFGVSQTKAIMTLVSG IPMGPPRLPLQKASREFTDSAEAKL KSLDFLSFTDLKDGNLEAGSTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NPL N-acetylneuraminate pyruvate lyase [ Homo sapiens (human) ]
• Official Symbol: NPL
• Synonyms: NPL; N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase); C1orf13, chromosome 1 open reading frame 13; N-acetylneuraminate lyase; DHDPS1; dihydrodipicolinate synthetase homolog 1 (E. coli); NPL1; NALase; sialic acid aldolase; sialate-pyruvate lyase; N-acetylneuraminic acid aldolase; dihydrodipicolinate synthetase homolog 1; NAL; C112; C1orf13; MGC61869; MGC149582
• Gene ID: 80896
• mRNA Refseq: NM_030769
• Protein Refseq: NP_110396
• MIM: 611412
• UniProt ID: Q9BXD5