Recombinant Human NRP1 protein, His-tagged

• Cat.No.: NRP1-806H
• Product Overview: Recombinant Human NRP1 protein (483-644aa) was fused to His-tag and expressed in E.coli.

Specification

• Description: This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Molecular Mass: 32 kDa
• AA Sequence: EWLQIDLGEEKIVRGIIIQGGKHRE NKVFMRKFKIGYSNNGSDWKMIMDD SKRKAKSFEGNNNYDTPELRTFPAL STRFIRIYPERATHGGLGLRMELLG CEVEAPTAGPTTPNGNLVDECDDDQ ANCHSGTGDDFQLTGGTTVLATEKP TVIDSTIQSGIK
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store for up to 12 months at -20 to -80 centigrade as lyophilized powder. Short-term storage: Store at 2-8 centigrade for (1-2 weeks). Long-term storage: Aliquot and store at -20 to -80 centigrade for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.
  If a different concentration is needed for your purposes please adjust the reconstitution
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: NRP1
• Official Symbol: NRP1
• Synonyms: NP1; NRP; BDCA4; CD304; VEGF165R
• Gene ID: 8829
• mRNA Refseq: NM_003873.7
• Protein Refseq: NP_003864.5
• MIM: 602069
• UniProt ID: O14786