Recombinant Human NRP1 Protein, His-tagged

• Cat.No.: NRP1-159H
• Product Overview: Recombinant Human NRP1 Protein with His tag was expressed in E. coli.

Specification

• Description: Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3 and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor. The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A. Neuropilin-1, a single pass transmembrane glycoprotein, was originally identified as a semaphorin receptor mediating axon growth cone collapse. In addition, neuropilin-1 is involved in axonal fasciculation, neuronal migration, dendritic guidance and repair of the adult nervous system. Neuropilin-1 is also an isoform-specific VEGF receptor on tumor and endothelial cells. Loss of neuropilin-1 function causes vascular remodeling and branching defects, which is compounded by additional loss of neuropilin-2 function.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~100 kDa
• AA Sequence: MFRNDKCGDTIKIESPGYLTSPGYP HSYHPSEKCEWLIQAPDPYQRIMIN FNPHFDLEDRDCKYDYVEVFDGENE NGHFRGKFCGKIAPPPVVSSGPFLF IKFVSDYETHGAGFSIRYEIFKRGP ECSQNYTTPSGVIKSPGFPEKYPNS LECTYIVFVPKMSEIILEFESFDLE PDSNPPGGMFCRYDRLEIWDGFPDV GPHIGRYCGQKTPGRIRSSSGILSM VFYTDSAIAKEGFSANYSVLQSSVS EDFKCMEALGMESGEIHSDQITASS QYSTNWSAERSRLNYPENGWTPGED SYREWIQVDLGLLRFVTAVGTQGAI SKETKKKYYVKTYKIDVSSNGEDWI TIKEGNKPVLFQGNTNPTDVVVAVF PKPLITRFVRIKPATWETGISMRFE VYGCKITDYPCSGMLGMVSGLISDS QITSSNQGDRNWMPENIRLVTSRSG WALPPAPHSYINEWLQIDLGEEKIV RGIIIQGGKHRENKVFMRKFKIGYS NNGSDWKMIMDDSKRKAKSFEGNNN YDTPELRTFPALSTRFIRIYPERAT HGGLGLRMELLGCEVEAPTAGPTTP NGNLVDECDDDQANCHSGTGDDFQL TGGTTVLATEKPTVIDSTIQSEFPT YGFNCEFGWGSHKTFCHWEHDNHVQ LKWSVLTSKTGPIQDHTGDGNFIYS QADENQKGKVARLVSPVVYSQNSAH CMTFWYHMSGSHVGTLRVKLRYQKP EEYDQLVWMAIGHQGDHWKEGRVLL HKSLKLYQVIFEGEIGKGNLGGIAV DDISINNHISQEDCAKPADLDKKNP EIKIDETGSTPGYEGEGEGDKNISR KPGNVLKTLDP
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: NRP1 neuropilin 1 [ Homo sapiens (human) ]
• Official Symbol: NRP1
• Synonyms: NRP1; neuropilin 1; neuropilin-1; CD304; NRP; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; NP1; BDCA4; DKFZp781F1414; DKFZp686A03134
• Gene ID: 8829
• mRNA Refseq: NM_001024628
• Protein Refseq: NP_001019799
• MIM: 602069
• UniProt ID: O14786