| Description : |
The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
129 kDa |
| AA Sequence : |
MFPAAPSPRTPGTGSRRGPLAGLGP GSTPRTASRKGLPLGSAVSSPVLFS PVGRRSSLSSRGTPTRMFPHHSITE SVNYDVKTFGSSLPVKVMEALTLAE VDDQLTINIDEGGWACLVCKEKLII WKIALSPITKLSVCKELQLPPSDFH WSADLVALSYSSPSGEAHSTQAVAV MVATREGSIRYWPSLAGEDTYTEAF VDSGGDKTYSFLTAVQGGSFILSSS GSQLIRLIPESSGKIHQHILPQGQG MLSGIGRKVSSLFGILSPSSDLTLS SVLWDRERSSFYSLTSSNISKWELD DSSEKHAYSWDINRALKENITDAIW GSESNYEAIKEGVNIRYLDFKQNCD GLVILAAAWHSADNPCLIYYSLITI EDNGCQMSDAVTVEVTQYNPPFQSE DLILCQLTVPNFSNQTAYLYNESAV YVCSTGTGKFSLPQEKIVFNAQGDS VLGAGACGGVPIIFSRNSGLVSITS RENVSILAEDLEGSLASSVAGPNSE SMIFETTTKNETIAQEDKIKLLKAA FLQYCRKDLGHAQMVVDELFSSHSD LDSDSELDRAVTQISVDLMDDYPAS DPRWAESVPEEAPGFSNTSLIILHQ LEDKMKAHSFLMDFIHQVGLFGRLG SFPVRGTPMATRLLLCEHAEKLSAA IVLKNHHSRLSDLVNTAILIALNKR EYEIPSNLTPADVFFREVSQVDTIC ECLLEHEEQVLRDAPMDSIEWAEVV INVNNILKDMLQAASHYRQNRNSLY RREESLEKEPEYVPWTATSGPGGIR TVIIRQHEIVLKVAYPQADSNLRNI VTEQLVALIDCFLDGYVSQLKSVDK SSNRERYDNLEMEYLQKRSDLLSPL LSLGQYLWAASLAEKYCDFDILVQM CEQTDNQSRLQRYMTQFADQNFSDF LFRWYLEKGKRGKLLSQPISQHGQL ANFLQAHEHLSWLHEINSQELEKAH ATLLGLANMETRYFAKKKTLLGLSK LAALASDFSEDMLQEKIEEMAEQER FLLHQETLPEQLLAEKQLNLSAMPV LTAPQLIGLYICEENRRANEYDFKK ALDLLEYIDEEEDININDLKLEILC KALQRDNWSSSDGKDDPIEVSKDSI FVKILQKLLKDGIQLSEYLPEVKDL LQADQLGSLKSNPYFEFVLKANYEY YVQGQITRTRPLEQKLISEEDLAAN DILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
