Recombinant Human PABPC1 Protein (1-370 aa), His-SUMO-tagged

• Cat.No.: PABPC1-707H
• Product Overview: Recombinant Human PABPC1 Protein (1-370 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.

Specification

• Description: Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in Cytoplasmic domain regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to Cytoplasmic domain RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the Cytoplasmic domain deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing prature stop codons; for the recognition of prature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 57.8 kDa
• Protein length: 1-370 aa
• AA Sequence: MNPSAPSYPMASLYVGDLHPDVTEA MLYEKFSPAGPILSIRVCRDMITRR SLGYAYVNFQQPADAERALDTMNFD VIKGKPVRIMWSQRDPSLRKSGVGN IFIKNLDKSIDNKALYDTFSAFGNI LSCKVVCDENGSKGYGFVHFETQEA AERAIEKMNGMLLNDRKVFVGRFKS RKEREAELGARAKEFTNVYIKNFGE DMDDERLKDLFGKFGPALSVKVMTD ESGKSKGFGFVSFERHEDAQKAVDE MNGKELNGKQIYVGRAQKKVERQTE LKRKFEQMKQDRITRYQGVNLYVKN LDDGIDDERLRKEFSPFGTITSAKV MMEGGRSKGFGFVCFSSPEEATKAV TEMNGRIVATKPLYVALAQR
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: PABPC1 poly(A) binding protein, cytoplasmic 1 [ Homo sapiens ]
• Official Symbol: PABPC1
• Synonyms: PABPC1; PABP1; PABPL1; PAB1; PABP; PABPC2
• Gene ID: 26986
• mRNA Refseq: NM_002568
• Protein Refseq: NP_002559
• MIM: 604679
• UniProt ID: P11940