Recombinant Human PAF1 protein, His-tagged

• Cat.No.: PAF1-2900H
• Product Overview: Recombinant Human PAF1 protein(166-531 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 166-531 aa
• AA Sequence: EEIYKDRDSQITAIEKTFEDAQKSI SQHYSKPRVTPVEVMPVFPDFKMWI NPCAQVIFDSDPAPKDTSGAAALEM MSQAMIRGMMDEEGNQFVAYFLPVE ETLKKRKRDQEEEMDYAPDDVYDYK IAREYNWNVKNKASKGYEENYFFIF REGDGVYYNELETRVRLSKRRAKAG VQSGTNALLVVKHRDMNEKELEAQE ARKAQLENHEPEEEEEEEMETEEKE AGGSDEEQEKGSSSEKEGSEDEHSG SESEREEGDRDEASDKSGSGEDESS EDEARAARDKEEIFGSDADSEDDAD SDDEDRGQAQGGSDNDSDSGSNGGG QRSRSHSRSASPFPSGSEHSAQEDG SEAAASDSSEADSDSD
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: PAF1 Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: PAF1
• Synonyms: PAF1; Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae); RNA polymerase II-associated factor 1 homolog; F23149_1; FLJ11123; PD2; pancreatic differentiation protein 2
• Gene ID: 54623
• mRNA Refseq: NM_001256826
• Protein Refseq: NP_001243755
• MIM: 610506
• UniProt ID: Q8N7H5

Reviews

09/29/2019

The PAF1 Protein's adaptability has significantly expanded the scope of my investigations and broadened the possibilities for my research.

10/23/2018

Its versatility and reliability make it a top choice for researchers in various fields.

11/20/2016

The PAF1 Protein is an exceptional product that fully meets my experimental needs.

Q&As

How does the PAF1 protein influence stem cell differentiation?

PAF1 regulates gene expression during stem cell differentiation, impacting cell fate decisions and developmental processes.

Are there any known interactions between PAF1 and other proteins that could be relevant in a clinical context?

PAF1 interacts with various proteins involved in transcriptional regulation, chromatin modification, and cellular signaling, highlighting its multifaceted role in cellular processes.

Can PAF1 protein levels be utilized as a diagnostic marker for autoimmune diseases?

Preliminary studies suggest that PAF1 expression may be altered in certain autoimmune conditions, warranting further investigation as a potential diagnostic marker.

In what ways does PAF1 participate in immune system regulation?

PAF1 is involved in the transcriptional regulation of immune-related genes, influencing immune system responses and inflammation.

How does PAF1 contribute to the maintenance of genomic stability?

PAF1 is involved in DNA repair mechanisms, and its proper function is crucial for maintaining genomic stability and preventing the accumulation of DNA damage.