Recombinant Human PAK1 Protein, GST-tagged

• Cat.No.: PAK1-821H
• Product Overview: Recombinant Human PAK1 protein Cdc42/Rac interactive binding region (AA 56-141) was expressed in E. coli with GST tag.

Specification

• Description: Members of the Rac/Rho subfamily of small GTPases or G-proteins are key regulators of numerous processes related to cytoskeletal dynamics. For example, Rac1/2 and cdc42 (and other closely related members) regulate cell migration, contraction and cell volume, among other features, through the control of the cortical actin cytoskeleton. Cdc42 is furthermore a major player in the establishment of a polarized phenotype in numerous specialized cell types whereas Rac is a critical regulator of NADPH oxidases and concomitant ROS production. In common with most other small G-proteins, cellular Rac and Cdc42 cycle between an inactive GDP-bound and an active GTP bound state. The CRIB domain of the Rac-effector PAK1 discriminates between these two conformations and can be used to isolate active Rac/Cdc42GTP complexes out of cell extracts.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: PBS
• Molecular Mass: 35 kDa
• Purity: > 90 % (SDS PAGE), 30-50 % degradation (Please note: effective concentration of full-length polypeptide takes into account protein degradation that commonly accounts for 30-50 % of total protein)
• Storage: Store at -80 centigrade, avoid freeze/thaw cycles., aliquot in convenient format upon first thawing.

Gene Information

• Gene Name: PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ]
• Official Symbol: PAK1
• Synonyms: PAKalpha; PAK1
• Gene ID: 5058
• mRNA Refseq: NM_001128620.1
• Protein Refseq: NP_001122092.1
• MIM: 602590
• UniProt ID: Q13153