Recombinant Human PARP14 Protein (F1208-E1387), His/Strep tagged

• Cat.No.: PARP14-0079H
• Product Overview: Recombinant Human 8His-StrepII-TEV-GG-PARP14(F1208-E1387) Protein was expressed in E. coli.

Specification

• Description: ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation. However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703'. Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription. In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation. Mono-ADP-ribosylates PARP9.
• Source: E. coli
• Species: Human
• Tag: His/Strep
• Form: Liquid
• Protein Length: F1208-E1387
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: PARP14 poly (ADP-ribose) polymerase family, member 14 [ Homo sapiens (human) ]
• Official Symbol: PARP14
• Synonyms: PARP14; poly (ADP-ribose) polymerase family, member 14; poly [ADP-ribose] polymerase 14; KIAA1268; pART8; collaborator of STAT6; B-aggressive lymphoma 2; b aggressive lymphoma protein 2; BAL2; PARP-14
• Gene ID: 54625
• mRNA Refseq: NM_017554
• Protein Refseq: NP_060024
• MIM: 610028
• UniProt ID: Q460N5