Recombinant Human PARP3 Protein, His & GST-tagged

• Cat.No.: PARP3-304H
• Product Overview: Recombinant C-terminal segment of human PARP3 isoform 1 (Q9Y6F1-1) (Met 1-Leu 533) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His & GST
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant human PARP3/GST chimera consists of 768 amino acids and has a calculated molecular mass of 87.8 kDa. It migrates as an approximately 95 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PARP3 poly (ADP-ribose) polymerase family, member 3 [ Homo sapiens ]
• Official Symbol: PARP3
• Synonyms: PARP3; poly (ADP-ribose) polymerase family, member 3; ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) like 3 , ADPRTL3; poly [ADP-ribose] polymerase 3; ADPRT3; hPARP 3; IRT1; NAD+ ADP ribosyltransferase 3; pADPRT 3; poly(ADP ribose) polymerase 3; poly(ADP ribose) synthetase 3; ADPRT-3; poly[ADP-ribose] synthase 3; NAD+ ADP-ribosyltransferase 3; poly(ADP-ribose) synthetase-3; poly[ADP-ribose] synthetase 3; NAD(+) ADP-ribosyltransferase 3; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2; poly (ADP-ribose) polymerase)-like 3; ADPRTL2; ADPRTL3; PADPRT-3
• Gene ID: 10039
• mRNA Refseq: NM_001003931
• Protein Refseq: NP_001003931
• MIM: 607726
• UniProt ID: Q9Y6F1
• Chromosome Location: 3p22.2-p21.1
• Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem

Reviews

01/25/2023

An additional advantage of selecting the PARP3 protein is the outstanding technical support offered by its manufacturer.

09/09/2019

Their unwavering commitment to customer satisfaction assures me that any challenges or queries I may encounter will be expertly resolved.

01/13/2016

The combination of the protein's excellence and the manufacturer's exceptional technical support equips me with the necessary resources to overcome experimental obstacles and achieve groundbreaking results.

Q&As

How does PARP3 interact with other proteins in the DNA repair pathway?

PARP3 interacts with various proteins involved in DNA repair, forming complexes that coordinate the repair process.

How does the dysregulation of PARP3 relate to cancer development?

Dysregulation of PARP3 can lead to genomic instability and impaired DNA repair, contributing to the development and progression of certain cancers.

Are there any diagnostic tools specifically detecting PARP3 activity?

While there are research tools for studying PARP3 activity in the laboratory, clinical diagnostic tools targeting PARP3 are still in the early stages of development.

Is PARP3 involved in the regulation of immune responses?

Emerging research suggests that PARP3 may play a role in immune regulation, and its modulation could potentially impact immune responses in diseases such as cancer.

Are there any clinical trials involving PARP3 inhibitors?

Some clinical trials are exploring the efficacy and safety of PARP inhibitors, and their potential application in cancers where PARP3 is implicated.