Recombinant Human PC Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PC-2370H
• Product Overview: PC MS Standard C13 and N15-labeled recombinant protein (NP_001035806) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 129.6 kDa
• AA Sequence: MLKFRTVHGGLRLLGIRRTSTAPAA SPNVRRLEYKPIKKVMVANRGEIAI RVFRACTELGIRTVAIYSEQDTGQM HRQKADEAYLIGRGLAPVQAYLHIP DIIKVAKENNVDAVHPGYGFLSERA DFAQACQDAGVRFIGPSPEVVRKMG DKVEARAIAIAAGVPVVPGTDAPIT SLHEAHEFSNTYGFPIIFKAAYGGG GRGMRVVHSYEELEENYTRAYSEAL AAFGNGALFVEKFIEKPRHIEVQIL GDQYGNILHLYERDCSIQRRHQKVV EIAPAAHLDPQLRTRLTSDSVKLAK QVGYENAGTVEFLVDRHGKHYFIEV NSRLQVEHTVTEEITDVDLVHAQIH VAEGRSLPDLGLRQENIRINGCAIQ CRVTTEDPARSFQPDTGRIEVFRSG EGMGIRLDNASAFQGAVISPHYDSL LVKVIAHGKDHPTAATKMSRALAEF RVRGVKTNIAFLQNVLNNQQFLAGT VDTQFIDENPELFQLRPAQNRAQKL LHYLGHVMVNGPTTPIPVKASPSPT DPVVPAVPIGPPPAGFRDILLREGP EGFARAVRNHPGLLLMDTTFRDAHQ SLLATRVRTHDLKKIAPYVAHNFSK LFSMENWGGATFDVAMRFLYECPWR RLQELRELIPNIPFQMLLRGANAVG YTNYPDNVVFKFCEVAKENGMDVFR VFDSLNYLPNMLLGMEAAGSAGGVV EAAISYTGDVADPSRTKYSLQYYMG LAEELVRAGTHILCIKDMAGLLKPT ACTMLVSSLRDRFPDLPLHIHTHDT SGAGVAAMLACAQAGADVVDVAADS MSGMTSQPSMGALVACTRGTPLDTE VPMERVFDYSEYWEGARGLYAAFDC TATMKSGNSDVYENEIPGGQYTNLH FQAHSMGLGSKFKEVKKAYVEANQM LGDLIKVTPSSKIVGDLAQFMVQNG LSRAEAEAQAEELSFPRSVVEFLQG YIGVPHGGFPEPFRSKVLKDLPRVE GRPGASLPPLDLQALEKELVDRHGE EVTPEDVLSAAMYPDVFAHFKDFTA TFGPLDSLNTRLFLQGPKIAEEFEV ELERGKTLHIKALAVSDLNRAGQRQ VFFELNGQLRSILVKDTQAMKEMHF HPKALKDVKGQIGAPMPGKVIDIKV VAGAKVAKGQPLCVLSAMKMETVVT SPMEGTVRKVHVTKDMTLEGDDLIL EIETRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PC pyruvate carboxylase [ Homo sapiens (human) ]
• Official Symbol: PC
• Synonyms: PC; pyruvate carboxylase; pyruvate carboxylase, mitochondrial; PCB; pyruvic carboxylase
• Gene ID: 5091
• mRNA Refseq: NM_001040716
• Protein Refseq: NP_001035806
• MIM: 608786
• UniProt ID: P11498

Reviews

06/19/2023

With the use of PC, the experiment is highly reproducible and convenient for data analysis.

01/11/2022

It is analyzed by X-ray crystallography or other crystallization techniques, and has good crystallization properties.

08/05/2021

It has a short half-life and high clearance.

Q&As

Is there a relationship between the structure and function of PC proteins?

The structure of the PC protein determines its ability and stability to bind to DNA. The study of its structure can help us understand its function and mechanism of action.

What are the therapeutic strategies for PC protein?

Therapeutic strategies for PC protein may include inhibiting its expression, blocking its binding to DNA, or regulating its associated signal transduction pathways. These methods may have potential applications in cancer treatment.

What is the specific role of PC protein in cancer?

In cancer, PC proteins may play a role by promoting processes such as DNA replication, repair, and regulation of the cell cycle, thus promoting tumor growth and spread. It could be a potential target for cancer treatment.

What factors should be considered for drug development targeting PC proteins?

Drug development targeting PC proteins needs to consider their specificity, safety, pharmacokinetic properties, and potential side effects. In addition, issues such as the dosage form, route of administration and design of clinical trials need to be considered.

How do PC proteins interact with other proteins?

The proteins can interact with a variety of other proteins, including DNA repair enzymes, cell cycle regulators, and more. These interactions allow it to regulate the regulatory processes of DNA replication, repair and the cell cycle.

Is there a correlation between PC protein expression and disease?

Recently published articles indicate that the expression level of PC protein is related to the occurrence and development of certain cancers. The highly expressed PC protein may promote tumor growth and spread and thus may be a potential therapeutic target.