Recombinant Human PCMT1 protein

• Cat.No.: PCMT1-195H
• Product Overview: Recombinant Human PCMT1 was expressed in E. coli.

Specification

• Description: This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: E. coli
• Species: Human
• Form: 25 mM Tris-Cl, pH 7.0, 150 mM NaCl, 5 mM 2-mercaptoethanol, and 10% glycerol.
• Notes: It is the human enzyme, but contains a single point mutation which appears to enhance the production yield (25-30 mg per liter of culture) with no effect on activity or stability.
• Concentration: 1.2 mg/ml (Bradford assay, normalized to absorbance-based concentration of pure PIMT at 280 nm)

Gene Information

• Gene Name: PCMT1 protein-L-isoaspartate (D-aspartate) O-methyltransferase [ Homo sapiens ]
• Official Symbol: PCMT1
• Synonyms: PCMT1; protein-L-isoaspartate (D-aspartate) O-methyltransferase; protein-L-isoaspartate(D-aspartate) O-methyltransferase; protein-beta-aspartate methyltransferase; L-isoaspartyl protein carboxyl methyltransferase; protein L-isoaspartyl/D-aspartyl methyltransferase; PIMT
• Gene ID: 5110
• mRNA Refseq: NM_001252049
• Protein Refseq: NP_001238978
• MIM: 176851
• UniProt ID: P22061
• Chromosome Location: 6q22.3-q24
• Function: protein-L-isoaspartate (D-aspartate) O-methyltransferase activity; transferase activity