Recombinant Human PDCD1 protein, His-tagged, low endotoxin (HPLC-verified)
Cat.No. : | PDCD1-892HL |
Product Overview : | Recombinant Human PDCD1 (Leu25-Gln167) protein (low endotoxin, HPLC-verified) was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293). |
- Specification
- Gene Information
- Related Products
Description : | Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer. |
Source : | HEK293 |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Leu25 |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Molecular Mass : | This protein has a calculated MW of 16.8 kDa. The reducing (R) protein migrates as 31-44 kDa in SDS-PAGE due to glycosylation. |
Protein length : | Leu25-Gln167 |
Endotoxin : | Less than 0.01 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | PDCD1 |
Official Symbol : | PDCD1 |
Synonyms : | PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l |
Gene ID : | 5133 |
mRNA Refseq : | NM_005018 |
Protein Refseq : | NP_005009 |
MIM : | 600244 |
UniProt ID : | Q15116 |
Products Types
◆ Recombinant Protein | ||
PDCD1-1003C | Active Recombinant Cynomolgus PDCD1 protein(Met1-Gln167), hFc&Avi-tagged, Biotinylated | +Inquiry |
PDCD1-703R | Recombinant Rat PDCD1 Protein | +Inquiry |
Pdcd1-160M | Active Recombinant Mouse Pdcd1 protein(Met1-Gln167), hFc-tagged | +Inquiry |
PDCD1-625M | Recombinant Mouse PDCD1 Protein, DDK/His-tagged | +Inquiry |
PDCD1-700M | Recombinant Mouse PDCD1 Protein, IgG2a Fc-tagged | +Inquiry |
◆ Lysates | ||
PDCD1-001RCL | Recombinant Rat PDCD1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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