Recombinant Human PDHA1, Gly & Pro tagged

• Cat.No.: PDHA1-266H
• Product Overview: Recombinant Human PDHA1 (P08559-1) (Phe30-Ser390), fused with two additional amino acids (Gly & Pro) at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Baculovirus-Insect cells
• Species: Human
• Tag: Gly & Pro
• Predicted N Terminal: Gly
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PDHA1 consists of 363 amino acids and has a calculated molecular mass of 40.4 kDa. The recombinant protein migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PDHA1 pyruvate dehydrogenase (lipoamide) alpha 1 [ Homo sapiens ]
• Official Symbol: PDHA1
• Gene ID: 5160
• mRNA Refseq: NM_000284
• Protein Refseq: NP_000275
• MIM: 300502
• UniProt ID: P08559
• Chromosome Location: Xp22.1

Reviews

06/27/2020

Incorporating the PDHA1 protein into your research will not only meet but exceed your expectations, enabling you to advance your scientific discoveries with confidence.

02/28/2019

Researchers can leverage the unique properties of the PDHA1 protein to gain insights into its biological functions and its impact on disease mechanisms.

07/10/2017

It has been shown to play a crucial role in multiple cellular processes, making it highly valuable for investigations into cell signaling, inflammation, and immune responses.

Q&As

What are the symptoms of PDHA1-related disorders?

Symptoms may include developmental delay, intellectual disability, muscle weakness, and movement disorders.

What are the treatment options for PDHA1-related disorders?

Treatment may involve dietary modifications, supplements, and supportive therapies to manage symptoms.

Are there any ongoing research efforts related to PDHA1 protein?

Yes, researchers are studying potential gene therapy approaches and novel treatments for PDHA1-related disorders.

How does the PDHA1 protein impact brain function?

The PDHA1 protein is critical for supplying energy to the brain, and mutations can lead to neurological impairments.

How does a mutation in the PDHA1 gene affect human health?

Mutations in the PDHA1 gene can lead to pyruvate dehydrogenase complex deficiency, resulting in neurological and developmental problems.