Recombinant Human PDK2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PDK2-3199H
• Product Overview: PDK2 MS Standard C13 and N15-labeled recombinant protein (NP_002602) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.2 kDa
• AA Sequence: MRWVWALLKNASLAGAPKYIEHFSK FSPSPLSMKQFLDFGSSNACEKTSF TFLRQELPVRLANIMKEINLLPDRV LSTPSVQLVQSWYVQSLLDIMEFLD KDPEDHRTLSQFTDALVTIRNRHND VVPTMAQGVLEYKDTYGDDPVSNQN IQYFLDRFYLSRISIRMLINQHTLI FDGSTNPAHPKHIGSIDPNCNVSEV VKDAYDMAKLLCDKYYMASPDLEIQ EINAANSKQPIHMVYVPSHLYHMLF ELFKNAMRATVESHESSLILPPIKV MVALGEEDLSIKMSDRGGGVPLRKI ERLFSYMYSTAPTPQPGTGGTPLAG FGYGLPISRLYAKYFQGDLQLFSME GFGTDAVIYLKALSTDSVERLPVYN KSAWRHYQTIQEAGDWCVPSTEPKN TSTYRVSTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PDK2 pyruvate dehydrogenase kinase 2 [ Homo sapiens (human) ]
• Official Symbol: PDK2
• Synonyms: PDK2; pyruvate dehydrogenase kinase, isozyme 2; pyruvate dehydrogenase kinase, isoenzyme 2; PDHK2; pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial
• Gene ID: 5164
• mRNA Refseq: NM_002611
• Protein Refseq: NP_002602
• MIM: 602525
• UniProt ID: Q15119