Recombinant Human Phosphatase And Tensin Homolog, GST-tagged

• Cat.No.: PTEN-1619H
• Product Overview: Full-length recombinant human PTEN was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Cat. No.: PTEN-1619H
• Description: PTEN is a tumor suppressor that is frequently mutated in a large number of cancers. PTEN has phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity and contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases.
• Applications: Phosphatase Assay; Western Blot
• Molecular Weight: 80 kDa
• Expression System: E. coli
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 435 nmol/min/mg
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Endometrial cancer; Focal adhesion; Glioma; Inositol phosphate metabolism; Melanoma; Pathways in cancer; Phosphatidylinositol signaling; Prostate cancer; Small cell lung cancer; Tight junction; p53 signaling pathway; Signaling in Immune system; Signalling by NGF; PIP3 activates AKT signaling

Gene Information

• Gene Name: PTEN phosphatase and tensin homolog [ Homo sapiens ]
• Official Symbol: PTEN
• Synonyms: PTEN; phosphatase and tensin homolog; BZS; DEC; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; MGC11227; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; OTTHUMP00000020032; phosphatase and tensin-like protein; mutated in multiple advanced cancers 1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; EC 3.1.3.16; EC 3.1.3.48; EC 3.1.3.67; Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog
• Gene ID: 5728
• mRNA Refseq: NM_000314
• Protein Refseq: NP_000305
• MIM: 601728
• UniProt ID: P60484
• Chromosome Location: 10q23
• Function: PDZ domain binding; hydrolase activity; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; platelet-derived growth factor receptor binding; protein binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; enzyme binding

Reviews

11/05/2022

Gel electrophoresis consistently displayed well-defined bands, affirming the product's purity and reliability.

01/18/2021

The insights gained from the product's application justified its cost, marking a prudent investment in our research endeavors.

12/20/2020

Comprehensive documentation streamlined the integration of the product into our experimental workflow.

Q&As

Apart from its lipid phosphatase activity, what additional function has been attributed to PTEN?

In addition to its lipid phosphatase activity, PTEN has an additional function known as a scaffolding protein that contributes to its tumor-suppressive role.

In which cellular compartment does PTEN primarily exert its lipid phosphatase function?

PTEN primarily exerts its lipid phosphatase function in the cytoplasm, where it regulates the PI3K/AKT pathway.

What is the significance of the FBXO22-mediated degradation of nuclear PTEN in promoting tumorigenesis?

The significance of FBXO22-mediated degradation of nuclear PTEN in promoting tumorigenesis involves the loss of PTEN's tumor-suppressive functions, leading to uncontrolled cell growth.

How is the expression of nuclear PTEN (核PTEN) regulated, especially in the context of tumorigenesis?

The expression of nuclear PTEN (核PTEN) is regulated, especially in the context of tumorigenesis, through mechanisms involving post-translational modifications and subcellular localization.

What differences are observed in the expression patterns of nuclear PTEN and cytoplasmic PTEN (浆PTEN) in various cancer processes?

Differences in expression patterns of nuclear PTEN and cytoplasmic PTEN in various cancer processes include alterations in nuclear PTEN levels contributing to tumorigenesis.

What is the classical function of the PTEN protein encoded by the PTEN gene?

The classical function of the PTEN protein is to regulate cell growth and prevent tumor formation by suppressing the PI3K/AKT signaling pathway.

What role does nuclear PTEN play in mediating the non-phosphatase-dependent function?

Nuclear PTEN plays a role in mediating the non-phosphatase-dependent function related to genomic stability and DNA repair.