Recombinant Human PIK3R1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PIK3R1-670H
• Product Overview: PIK3R1 MS Standard C13 and N15-labeled recombinant protein (NP_852556) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 53.3 kDa
• AA Sequence: MYNTVWNMEDLDLEYAKTDINCGTD LMFYIEMDPPALPPKPPKPTTVANN GMNNNMSLQDAEWYWGDISREEVNE KLRDTADGTFLVRDASTKMHGDYTL TLRKGGNNKLIKIFHRDGKYGFSDP LTFSSVVELINHYRNESLAQYNPKL DVKLLYPVSKYQQDQVVKEDNIEAV GKKLHEYNTQFQEKSREYDRLYEEY TRTSQEIQMKRTAIEAFNETIKIFE EQCQTQERYSKEYIEKFKREGNEKE IQRIMHNYDKLKSRISEIIDSRRRL EEDLKKQAAEYREIDKRMNSIKPDL IQLRKTRDQYLMWLTQKGVRQKKLN EWLGNENTEDQYSLVEDDEDLPHHD EKTWNVGSSNRNKAENLLRGKRDGT FLVRESSKQGCYACSVVVDGEVKHC VINKTATGYGFAEPYNLYSSLKELV LHYQHTSLVQHNDSLNVTLAYPVYA QQRRTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PIK3R1 phosphoinositide-3-kinase regulatory subunit 1 [ Homo sapiens (human) ]
• Official Symbol: PIK3R1
• Synonyms: PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphatidylinositol 3-kinase regulatory subunit alpha; GRB1; p85; p85 ALPHA; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); p85-ALPHA
• Gene ID: 5295
• mRNA Refseq: NM_181504
• Protein Refseq: NP_852556
• MIM: 171833
• UniProt ID: P27986