Recombinant Human PLCZ1 Protein, His-tagged

• Cat.No.: PLCZ1-2596H
• Product Overview: Recombinant Human PLCZ1 Protein (Ser469-Thr572) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 15.4 kDa
  Accurate Molecular Mass: 13/15 kDa
• Protein length: Ser469-Thr572
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PLCZ1 phospholipase C, zeta 1 [ Homo sapiens (human) ]
• Official Symbol: PLCZ1
• Synonyms: PLCZ1; phospholipase C, zeta 1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1; NYD SP27; PLCzeta; PLC-zeta-1; phospholipase C-zeta-1; PI-phospholipase C zeta 1; testis-development protein NYD-SP27; testis-development related NYD-SP27; phosphoinositide phospholipase C-zeta-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1; NYD-SP27; MGC149685
• Gene ID: 89869
• mRNA Refseq: NM_033123
• Protein Refseq: NP_149114
• MIM: 608075
• UniProt ID: Q86YW0