Recombinant Human PLEKHO1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: PLEKHO1-302H
• Product Overview: PLEKHO1 MS Standard C13 and N15-labeled recombinant protein (NP_057358) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.2 kDa
• AA Sequence: MMKKNNSAKRGPQDGNQQPAPPEKV GWVRKFCGKGIFREIWKNRYVVLKG DQLYISEKEVKDEKNIQEVFDLSDY EKCEELRKSKSRSKKNHSKFTLAHS KQPGNTAPNLIFLAVSPEEKESWIN ALNSAITRAKNRILDEVTVEEDSYL AHPTRDRAKIQHSRRPPTRGHLMAV ASTSTSDGMLTLDLIQEEDPSPEEP TSCAESFRVDLDKSVAQLAGSRRRA DSDRIQPSADRASSLSRPWEKTDKG ATYTPQAPKKLTPTEKGRCASLEEI LSQRDAASARTLQLRAEEPPTPALP NPGQLSRIQDLVARKLEETQELLAE VQGLGDGKRKAKDPPRSPPDSESEQ LLLETERLLGEASSNWSQAKRVLQE VRELRDLYRQMDLQTPDSHLRQTTP HSQYRKSLMTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PLEKHO1 pleckstrin homology domain containing, family O member 1 [ Homo sapiens (human) ]
• Official Symbol: PLEKHO1
• Synonyms: PLEKHO1; pleckstrin homology domain containing, family O member 1; pleckstrin homology domain-containing family O member 1; CKIP 1; OC120; JBP; 2810052M02Rik; C-Jun-binding protein; CK2-interacting protein 1; casein kinase 2-interacting protein 1; PH domain-containing family O member 1; CK2 interacting protein 1; HQ0024c protein; osteoclast maturation-associated gene 120 protein; CKIP-1; RP11-458I7.3
• Gene ID: 51177
• mRNA Refseq: NM_016274
• Protein Refseq: NP_057358
• MIM: 608335
• UniProt ID: Q53GL0