Recombinant Human PLIN2 Protein (444 AA), N-8×His tagged

• Cat.No.: PLIN2-23H
• Product Overview: Recombinant Human PLIN2 Protein with N-8×His-tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-8×His
• Form: Lyophilized
• Molecular Mass: 49,03 kDa (calculated)
• Protein Length: 444 AA
• AA Sequence: MKHHHHHHASVAVDPQPSVVTRVVN LPLVSSTYDLMSSAYLSTKDQYPYL KSVCEMAENGVKTITSVAMTSALPI IQKLEPQIAVANTYACKGLDRIEER LPILNQPSTQIVANAKGAVTGAKDA VTTTVTGAKDSVASTITGVMDKTKG AVTGSVEKTKSVVSGSINTVLGSRM MQLVSSGVENALTKSELLVEQYLPL TEEELEKEAKKVEGFDLVQKPSYYV RLGSLSTKLHSRAYQQALSRVKEAK QKSQQTISQLHSTVHLIEFARKNVY SANQKIQDAQDKLYLSWVEWKRSIG YDDTDESHCAEHIESRTLAIARNLT QQLQTTCHTLLSNIQGVPQNIQDQA KHMGVMAGDIYSVFRNAASFKEVSD SLLTSSKGQLQKMKESLDDVMDYLV NNTPLNWLVGPFYPQLTESQNAQDQ GAEMDKSSQETQRSEHKTH
• Purity: > 95%
• Applications: WB
• Quality Control Test: BCA to determine quantity of the protein.
  SDS PAGE to determine purity of the protein.
• Notes: This product is intended for research use only.
• Storage: Store the lyophilized protein at –80 centigrade. Lyophilized protein remains stable until the expiry date when stored at –80 centigrade. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 centigrade for long term storage. Reconstituted protein can be stored at 4 centigrade for a week.
• Concentration: 0.5 mg/mL
• Storage Buffer: Filtered (0.4 μm) in 20mM Tris buffer, 20mM NaCl, pH 7.5
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
• Shipping: At ambient temperature.

Gene Information

• Gene Name: PLIN2 perilipin 2 [ Homo sapiens (human) ]
• Official Symbol: PLIN2
• Synonyms: PLIN2; perilipin 2; ADFP; ADRP; perilipin-2; adipophilin; adipose differentiation-related protein
• Gene ID: 123
• mRNA Refseq: NM_001122
• Protein Refseq: NP_001113
• MIM: 103195
• UniProt ID: Q99541