Recombinant Human POLE protein
Cat.No. : | POLE-1522H |
Product Overview : | Recombinant human DNA polymerase epsilon catalytic fragment (wild type, exo-proficient) expressed in E. coli. |
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Description : | DNA polymerase epsilon is a member of B family DNA polymerases. It is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have recently been found associated with colorectal cancer. |
Source : | E. coli |
Species : | Human |
Form : | 50 mM Tris-HCl (pH7.5), 500 mM NaCl, and 10% glycerol. |
Molecular Mass : | 168 kDa |
Usage : | Suggested reaction buffer (not included): 50 mM Tris-HCl (pH7.4), 8 mM MgCl2, 1 mM DTT, 10% glycerol, 200 M dNTPs, 100 nM DNA primer-template, and, 2 nM -10nM pol epsilon. Suggested dilution buffer (not included): 50 mM Tris-HCl (pH 7.5), 5 mM-mercaptoethanol, 50% glycerol. Reaction temperature: 37 centigrade Note: Reaction can be stopped by adding equal volume of 95% formamide. |
Storage : | Stable for 2 years at -70 centigrade from date of shipment. Please aliquot to avoid repeated freezing and thawing. |
Gene Name : | POLE polymerase (DNA directed), epsilon [ Homo sapiens ] |
Official Symbol : | POLE |
Synonyms : | POLE; polymerase (DNA directed), epsilon; DNA polymerase epsilon catalytic subunit A; POLE1; DNA polymerase II subunit A; FLJ21434; DKFZp434F222; |
Gene ID : | 5426 |
mRNA Refseq : | NM_006231 |
Protein Refseq : | NP_006222 |
MIM : | 174762 |
UniProt ID : | Q07864 |
Products Types
◆ Recombinant Protein | ||
POLE-6913Z | Recombinant Zebrafish POLE | +Inquiry |
◆ Lysates | ||
POLE-1390HCL | Recombinant Human POLE cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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