Recombinant Human PRAME protein, His-tagged

• Cat.No.: PRAME-3368H
• Product Overview: Recombinant Human PRAME protein(P78395)(1-509aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 61.9 kDa
• Protein length: 1-509aa
• AA Sequence: MERRRLWGSIQSRYISMSVWTSPRR LVELAGQSLLKDEALAIAALELLPR ELFPPLFMAAFDGRHSQTLKAMVQA WPFTCLPLGVLMKGQHLHLETFKAV LDGLDVLLAQEVRPRRWKLQVLDLR KNSHQDFWTVWSGNRASLYSFPEPE AAQPMTKKRKVDGLSTEAEQPFIPV EVLVDLFLKEGACDELFSYLIEKVK RKKNVLRLCCKKLKIFAMPMQDIKM ILKMVQLDSIEDLEVTCTWKLPTLA KFSPYLGQMINLRRLLLSHIHASSY ISPEKEEQYIAQFTSQFLSLQCLQA LYVDSLFFLRGRLDQLLRHVMNPLE TLSITNCRLSEGDVMHLSQSPSVSQ LSVLSLSGVMLTDVSPEPLQALLER ASATLQDLVFDECGITDDQLLALLP SLSHCSQLTTLSFYGNSISISALQS LLQHLIGLSNLTHVLYPVPLESYED IHGTLHLERLAYLHARLRELLCELG RPSMVWLSANPCPHCGDRTFYDPEP ILCPCFMPN
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Gene Information

• Gene Name: PRAME preferentially expressed antigen in melanoma [ Homo sapiens ]
• Official Symbol: PRAME
• Synonyms: PRAME; preferentially expressed antigen in melanoma; MAPE; melanoma antigen preferentially expressed in tumors; cancer/testis antigen 130; CT130; OIP-4; opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen of melanoma; OIP4
• Gene ID: 23532
• mRNA Refseq: NM_006115
• Protein Refseq: NP_006106
• MIM: 606021
• UniProt ID: P78395