Recombinant Human PRAME Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PRAME-1474H
• Product Overview: PRAME MS Standard C13 and N15-labeled recombinant protein (NP_996838) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 57.9 kDa
• AA Sequence: MERRRLRGSIQSRYISMSVWTSPRR LVELAGQSLLKDEALAIAALELLPR ELFPPLFMAAFDGRHSQTLKAMVQA WPFTCLPLGVLMKGQHLHLETFKAV LDGLDVLLAQEVRPRRWKLQVLDLR KNSHQDFWTVWSGNRASLYSFPEPE AAQPMTKKRKVDGLSTEAEQPFIPV EVLVDLFLKEGACDELFSYLIEKVK RKKNVLRLCCKKLKIFAMPMQDIKM ILKMVQLDSIEDLEVTCTWKLPTLA KFSPYLGQMINLRRLLLSHIHASSY ISPEKEEQYIAQFTSQFLSLQCLQA LYVDSLFFLRGRLDQLLRHVMNPLE TLSITNCRLSEGDVMHLSQSPSVSQ LSVLSLSGVMLTDVSPEPLQALLER ASATLQDLVFDECGITDDQLLALLP SLSHCSQLTTLSFYGNSISISALQS LLQHLIGLSNLTHVLYPVPLESYED IHGTLHLERLAYLHARLRELLCELG RPSMVWLSANPCPHCGDRTFYDPEP ILCPCFMPNTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PRAME preferentially expressed antigen in melanoma [ Homo sapiens (human) ]
• Official Symbol: PRAME
• Synonyms: PRAME; preferentially expressed antigen in melanoma; MAPE; melanoma antigen preferentially expressed in tumors; cancer/testis antigen 130; CT130; OIP-4; opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen of melanoma; OIP4
• Gene ID: 23532
• mRNA Refseq: NM_206955
• Protein Refseq: NP_996838
• MIM: 606021
• UniProt ID: P78395